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CHEMISTRY : Chromatography
Solvents: ETHANOL
| PROJECT CODE: | 04.01c |
| SECTION: | CHROMATOGRAPHY |
| PROJECT TITLE: | Solvents Characteristics : ETHANOL (C2H5OH) |
| RELEASE DATE: | 01 Jan 99 - 03 Jan 99 |
| LAST UPDATE: | 2 Sep 2009 |
| VERSION HISTORY: | 1.0 - First release 1.32 - (01Jan, 03Jan, 10Jan, 30Jan, 07Feb) 1.4 - Revision of text and formatting. (2-Sep-2009) |
| Solvent: | Ethanol - CH3-CH2.OH | |||||
|---|---|---|---|---|---|---|
| Concentration | 80% | 70% | 60% | 50% | 40% | 30% |
| Flow Rate: | 20% in 10mins 40% in 29mins 60% in 60mins |
80% in 1:45hrs | 20% in 10mins 50% in 43mins 60% in 60mins |
40% in 25mins 78% in 1:45hrs 90% in 2:15hrs |
20% in 9mins 50% in 45mins 60% in 60mins |
20% in 9mins 50% in 45mins 65% in 60mins |
| Solubility: | 9/10 Excellent, dyes move with level of solvent |
8½/10 Excellent, very faint spot was left |
8/10 Very Good |
8/10 Very Good, quite dense and dark |
6/10 Quite Good |
3/10 Very faint but visible |
| Separation of dyes: | 0/10 No separation at all. |
3/10 Did not seperated, one zone with huge overlapping. |
7/10 Good, roundish zones seperated by about 1mm |
7/10 Good, roundish zones but nearly touching |
8/10 Very Good, two seperate slightl enlongated zones |
5/10 Quite good, but remains as long streaks |
| Allocated Time: | 50 - 60 mins | 1¾ - 2 hours | 2 - 3 hours | 2 - 2½ hours | 90 - 100 mins | 90 - 100 mins |
| Score: | 26% | 54% | 92% | 88% | 82% | 20% |
| Concentration | 80% | 70% | 60% | 50% | 40% | 30% |
| Comments: | Although its great solubility, there is no separation of the two colours. The dyes move with the edge of the solvent rising in the strip, followed by a traling tail of both colours. If left for long, the dyes rapidly move to the very top of the solvent, forming just a insignificant line at the edge. To avoid this a short time of about 1hour is proposed. | The dense colours on teh strip, and the faint application point left behind, prooves the excellent solubility. However separation was n ot complete, with the impression that the 2 zones merge into each other forming a single enlongated thick zone with a violet tip at the top, a cyan tail at the bottom, and a mixture at the centre. Propably the cyan dye moved further near the violet dye with an increase in concentration, merging the two zones into one. | Excellent results, same separation as with 50%, with a distance of about 2mm from the two seperate roundish zones. This could be improved if leaving for 3hrs or so. The zone colours are well formed and dense especially the Cyan one. | Excellent results both in separation and ammount of dye seperated (solubility). Two seperate roundish zones were formed, seperated by just about 2mm. This space was shown to increase if left for about 3hrs. Both colours are well visible due to good solubility. | Excellent separation with distinct enlongated zones seperated by about 8mm from each other. However the solubility is rather low, seperating a relatively low ammount from the ink drop. Said that, both colours are well visible. | Good separation forming enlongated thin zones at a distance of about 10 mm from each other. However due to the low concentration of ethanol very little ink was dissolved resulting in faint separation zones, - the cyan one hardly visibly. |
| BEST Separation: | 60% Ethanol - 120-140mins |
| For wider separation: | 40% Ethanol - 90-100 mins |
| Artistic effect - flame shape with merging colours: | 70% Ethanol - 120mins |
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